Covers the use of gas chromatographic techniques in chemical analysis. The course starts with a brief review of separation theory applied to gas chromatographic separations. Subsequent sections of the course cover topics related to more advanced aspects of gas chromatography. Topics include maintenance of gas chromatographs, logical troubleshooting steps in gas chromatography, optimizing gas chromatographic separations, headspace analysis and fast GC. Laboratory exercises provide practical training of the principles covered in the lectures.
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Upon successful completion of this course, the student will be able to:
Demonstrate a thorough knowledge of the general theory of chromatography.
Explain the general theory of gas chromatography.
Compare method development strategies for gas chromatography.
Recommend appropriate steps to optimise separations in gas chromatography.
Recommend logical troubleshooting steps for solving common problems encountered in gas chromatography.
Plan and implement maintenance operations for GC components including inlets, columns and detectors.
Develop optimal conditions for split and splitless injection techniques in GC.
Identify different sample introduction techniques including headspace analysis, purge and trap analysis and solid-phase microextraction.
Demonstrate a thorough knowledge of instrumentation validation in gas chromatography.
Develop strategies for quantitative and qualitative analysis in GC using common software packages.
Evaluate the use of derivatisation techniques in gas chromatography.
Explain the importance of retention time locking techniques and fast GC for gas chromatographic analysis.
Effective as of Winter 2007
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