This course provides instruction in forensic biology with a focus on current short tandem repeat (STR) technologies. The design of the course follows the logical progression of a forensic DNA case by including instruction in: evidence gathering and preservation techniques, the identification of biological evidence including presumptive and confirmatory testing, the extraction, quantitation and PCR amplification analysis strategies for common types of biological evidence (bodily fluids, hard and soft tissues and "touch" DNA), and the interpretation methods utilized for single-source profiles, mixed profiles, parentage and kinship scenarios. Each module also includes practical interpretation exercises based on real case work scenarios in order to underpin the theoretical aspects of this applied forensic discipline.
Admission to a Forensic credential program or permission of the Program Coordinator.
For department approval, please contact the Program Assistants Fiona or Julia @ [email protected].. For details on the textbook required, please check: Hybrid course with online delivery Sept 9 - Dec 6; in-class on Fridays Oct 18, Nov 1, Nov 8, Nov 29.
Upon successful completion of this course, the student will be able to:
Compare and contrast “historical” and contemporary DNA Typing technologies.
Select proper evidence collection and handling techniques from a given crime scene (from the perspective of the forensic biologist).
Determine proper evidence recovery techniques for evidentiary items submitted to the laboratory for forensic DNA analysis.
Justify the choice of presumptive and/or confirmatory tests for specific types of DNA evidence.
Interpret the results of presumptive and/or confirmatory tests for biological fluids.
Explain proper DNA extraction (purification) methods.
Justify the choice of DNA purification method, consistent with the type of DNA evidence.
Differentiate the current DNA quantitation methods for DNA evidence.
Interpret DNA quantitation results with respect to subsequent DNA analysis methods.
Contrast the PCR-STR’s methods utilized for human identification applications.
Evaluate the quality of a single-source and mixed STR profiles for human identification applications.
Apply the statistical analysis methods pertinent to a forensic DNA analysis.
Interpret a single-source STR profile for human identification applications.
Interpret a mixed STR profile for human identification applications.
Conduct Random Match Probability, Likelihood Ratio, and Combined Probability of Inclusion calculations.
Discuss the fundamentals of DNA separation and detection utilizing a genetic analyzer.
Interpret challenging DNA profiles that are the result of degraded, low template or mixed DNA.
Justify alternative applications of STR technologies including the use in kinship and parentage cases.
Discuss the role of quality assurance in the lab and how it can help prevent or recognize laboratory errors.
Outline the Combined DNA Index System (CODIS) and explain the importance of DNA databases.
Effective as of Spring/Summer 2015
FSCT 8150 is offered as a part of the following programs:
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